Agilent

Filters:

• IsWellAboveBG
• Saturated spot This removes all spots where the signal is to high to measure accurately.
• Control spots
• Spots flagged by Feature Extraction
  • gIsFeatNonUnifOL
  • rIsFeatNonUnifOL
  • gIsBGNonUnifOL
  • rIsBGNonUnifOL
  • gIsFeatPopnOL
  • rIsFeatPopnOL
  • gIsBGPopnOL
  • rIsBGPopnOL

Normalisation:

• One colour arrays: Quantile Normalization
• Two colour arrays: Lowess